Journal: iScience

Article Title: Design of a gradient-rheotaxis microfluidic chip for sorting of high-quality Sperm with progressive motility

doi: 10.1016/j.isci.2023.107356

Figure Lengend Snippet:

Article Snippet: Comsol 5.5 , COMSOL Multiphysics® Simulation Software , https://www.comsol.com/comsol-multiphysics.

Techniques: Recombinant, Staining, Software

Journal: medRxiv

Article Title: A Cascaded Droplet Microfluidic Platform Enables High-throughput Single Cell Antibiotic Susceptibility Testing at Scale

doi: 10.1101/2021.06.25.21259551

Figure Lengend Snippet: (A)(i) In the absence of barrierplugs, picodroplets can disperse as they flow through the incubation channel and cause significant mixing among picodroplet groups. (ii) Such dispersion and mixing can be fluorescently detected as picodroplet groups with distinct fluorophore concentrations would yield a fluorescence trace showing mixed peaks ( i . e ., picodroplets) with distinct avalanche photodiode (APD) photon counts ( i . e ., fluorescence intensities). (B)(i) Two-dimensional water-oil multiphase flow CFD simulation reveals that, within an oil-filled, 500-µm-wide microchannel (white) and under pressure-driven flow, a water barrierplug (black ellipse, 1000 μm × 500 μm semiaxes) that seals the entire width of the channel flows faster than a downstream water picodroplet (black circle, 25 μm in diameter, indicated by black arrows) that is positioned at the wall of the channel, as indicated by the decreasing distance between them over time (t = 0, 1, 2, and 3 s). (ii) Experimental observation within the incubation channel of a SCALe-AST device corroborates with the simulation, as a barrierplug indeed catch up to picodroplets at the channel wall over time (t = 0, 3, 6, and 9 s). (C)(i) In the presence of barrierplugs, which prevent dispersion of picodroplets, picodroplet groups therefore become tightly packed and well separated from each other. (ii) Well separated picodroplet groups due to the addition of barrierplugs can be fluorescently detected as picodroplet groups with distinct fluorophore concentrations could now yield a fluorescence trace showing well separated groups of peaks with distinct photon counts.

Article Snippet: Two-dimensional multiphase fluid simulations of a big barrierplug and a picodroplet flowing in a straight microfluidic channel were conducted with a CFD package (COMSOL Multiphysics®, version 5.5, COMSOL Inc.).

Techniques: Incubation, Dispersion, Fluorescence

Journal: ACS Sensors

Article Title: Flow Rate-Independent Multiscale Liquid Biopsy for Precision Oncology

doi: 10.1021/acssensors.2c02577

Figure Lengend Snippet: Comparative (semiquantitative) multiphysics simulations. (a) shows the effect of the flow rate on the capture of particles on the mesh (cross-section). The capture efficiency decreases progressively as the flow rate increases. The values observed are based on at least three individual measurements in the same conditions. (b) shows the effect of restrictions in the vicinity of the mesh (cross-section). If the mesh is too close to a diameter restriction, the velocity increases locally, thereby negatively impacting the capture efficiency.

Article Snippet: Comparative (semiquantitative) multiphysics simulations (COMSOL Multiphysics 5.5) were performed to optimize the device ( Figure ).

Techniques: